1. When comparing one chromatography column to another which parameter might you be most interested in if you wanted the best separation of two components?
a) theoretical plates of the two columns
b) the retention time of the two columns
c) the peak area to sensitivity of the two columns
d) the integral detector response curve of each column
2-6 For these questions refer to Figure 1.
2. What is the retention time, tR for the B peak in the chromatogram below?
3. What is the the retention volume for the B peak if the flow of the carrier gas is 21 mL/min?
4. Based on peak C, show your calculations
of the number of theoretical plates for this column.
5. If the column length is 8 feet, what is the HETP in mm.
6. What is the resolution between
peaks A and B?